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. 2016 Sep 15;7:12847. doi: 10.1038/ncomms12847

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Extinction coefficient (ɛ), quantum yield (Φ), and brightness (product of ɛ and Φ) were determined for all incorrectly inferred ancestral FP proteins and compared to the properties of the true ancestral protein at each node and reported as a function of percent error. Dark brown bars are PAML with a gamma distribution, light brown bars are FastML with a gamma distribution, yellow bars are PAML, light grey bars are PhyloBayes with a gamma distribution, and dark grey bars are maximum parsimony. Single and double asterisks represent confidence at 95% and 99% levels, respectively, and are coloured according to the respective procedure that has significantly less error.