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. 2016 Sep 15;7:12840. doi: 10.1038/ncomms12840

Figure 3. PHD inhibition augments cold-induced activation of mTRPA1 via an enhanced response to ROS.

Figure 3

mTRPA1-expressing cells were exposed to cold (a–c) or 10 μM H2O2 (d) and analysed using Ca2+ imaging experiments or inside-out patch-clamp recordings. (a) Representative traces of cold-induced [Ca2+]i responses in mTRPA1-expressing cells pretreated with or without DMOG (100 μM) for 2 h (n=8–19 cells) in the presence or absence of 3 mM PBN, and (b) statistical analysis (n=39–96 cells). **P<0.01 versus non-treated mTRPA1 (white bar); ###P<0.001 versus DMOG-pretreated mTRPA1 (magenta bar); one-way ANOVA with Tukey's post hoc test. (c) Relative open probability (NPO) of mTRPA1 in inside-out patches in the presence 0.1 μM H2O2 at 16 °C from a mTRPA1-expressing cell pretreated with or without DMOG (100 μM) for 2 h. n=8–11. *P<0.05; unpaired t test. Membrane potential was −60 mV. (d) Statistical analysis of the H2O2-evoked [Ca2+]i responses in mTRPA1-expressing cells pretreated with or without DMOG (100 μM) for 2 h. n=10–12. *P<0.05; unpaired t test. All data are expressed as mean±s.e.m.