Figure 5. L-OHP or DMO augments the H₂O₂-evoked [Ca²⁺]_i increase in mouse DRG neurons.

Shown are representative traces of 100 μM H₂O₂-induced [Ca²⁺]_i responses in DRG neurons derived from wild-type (WT, a–c) or TRPA1-KO (e–g) mice pretreated with vehicle (a,e; control), 100 μM L-OHP (b,f) or 30 μM DMO (c,g) for 2 h. Statistical analysis of the H₂O₂-evoked [Ca²⁺]_i increase is shown in d (WT-DRG; n=5–6) and h (TRPA1-KO-DRG; n=4–5). *P<0.05 versus each control (Ctrl); one-way ANOVA with Tukey's post hoc test. AITC, 100 μM; capsaicin, 0.3 μM; KCl, 50 mM. No difference was detected in the AITC-evoked [Ca²⁺]_i increase among groups of WT-DRG neurons (relative ΔRatio versus vehicle: L-OHP, 1.02±0.06; DMO, 1.14±0.13). All data are expressed as mean±s.e.m.