Table 1. Parameters of hTRPA1-WT and hTRPA1-P394A activity in the cold and/or H₂O₂ stimulation.

	26 °C		16 °C
	Basal	H2O2 (0.1 μM)	Basal	H2O2 (0.1 μM)
hTRPA1-WT
Conductance (pS)	37.2±1.7	34.2±1.7	41.7±2.9	43.6±3.2
NPO	0.19±0.07	0.15±0.04	0.31±0.08	0.30±0.07
hTRPA1-P394A
Conductance (pS)	40.2±1.8	42.2±1.6	47.2±3.5	51.2±2.3**
NPO	0.46±0.19	0.61±0.31	0.94±0.36*	1.43±0.52***,†

Single-channel conductance and open probability (NP_O) of single-channel currents were measured in hTRPA1-WT- or hTRPA1-P394A-expressing cells at 26 or 16 °C in the presence or absence of H₂O₂ (0.1 μM) using inside-out patches at a membrane potential of −60 mV as shown in Fig. 1e–g. n=13–14.

*P<0.05.

**P<0.01.

***P<0.001 versus data from the same channel at 26 °C in the absence of H₂O₂;

†P<0.01 versus hTRPA1-WT under the same condition; two-way repeated measures ANOVA with Bonferroni's post hoc test. All data are expressed as mean±s.e.m.