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. 2016 Sep 19;14:78. doi: 10.1186/s12915-016-0305-y

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© League and Hillyer. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Larval hemocytes associate with the eighth abdominal segment tracheal tufts and are distinct from the segmental hemocyte bands. a Posterior portion of the dorsal abdomen of a dissected larva showing the trachea of the seventh and eighth abdominal segments under bright-field and fluorescence illumination. A dense mass of tracheoles known collectively as the tracheal tufts (TT) extend ventrally from the dorsal longitudinal tracheal trunks (T). Hemocytes (CM-DiI; red) bind to the tracheal tufts in high numbers. b, c EDF (b) and 3D volume view (c) images of a portion of the seventh and eighth abdominal segments of a larva. Tracheal tuft hemocytes (rectangles) lie ventral to the dorsal segmental hemocytes. Directional arrows: A anterior, P posterior, D dorsal, V ventral, L lateral