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. 2016 May 11;24(10):1453–1459. doi: 10.1038/ejhg.2016.40

Figure 4.

Figure 4

PHF6 mediates the expression of rDNA coding and non-coding transcripts. (a) qRT-PCR analysis (n=8) of RNA isolated from HEK 293T cell lines expressing empty vector or one of two PHF6 overexpression constructs (PHF6-NTAP, PHF6-CTAP). All Ct values were normalized to GAPDH amplification and fold changes were calculated relative to the empty vector dCt value for all treatments. (b) qRT-PCR analysis (n=6) of RNA isolated from wild-type HEK 293T cells that were treated with 0.5 μg/ml Act D (2 h), 25 μg/ml DRB (2 h), 10 ng/ml trichostatin A (24 h), or 50 μM 5-azacytidine (24 h). The y axis is logarithmic (base 10). Bars represent SE (*P<0.05, **P<0.01, two-tailed Student's t-test). (c) Proposed model to explain how PHF6 overexpression could lead to the arrest of rRNA transcription and RNA Pol I-mediated expression of non-coding IGS transcripts. See Supplementary Figure S4 for additional controls.