TABLE III. Performance of the mpt64 real-time polymerase chain reaction (PCR) assay based on valid culture identification results.
| Culture (n = 1,451) | ||||||||
|---|---|---|---|---|---|---|---|---|
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| Real-time PCR | Positive | Negative | Total | Sensitivity (95% CI) | Specificity (95% CI) | PPV (95% CI) | NPV (95% CI) | Accuracy |
| Positive | 1,347 | 4* | 1,351 | 99.7% (99.2-99.9) | 96% (90.1-98.9) | 99.7% (99.2-99.9) | 96% (90.1-98.9) | 99.4% |
| Negative | 4 | 96 | 100 | |||||
NPV: negative predictive value; PPV: positive predictive value; *: all were NTM isolates: one Mycobacterium avium/intracellulare, one M. kansasii, one M. chelonae and one achromogen rapid grower.