TABLE I. Oligonucleotides used for multilocus sequence typing.
| Gene target | Primer name | Sequence (5’ to 3’) | Amplicon size (bp) | Nt location | AT (ºC) | Reference |
|---|---|---|---|---|---|---|
| acsA | acsA-F | GCCACACCTACATCGTCTAT | 520 | 935-1454bp | 53 | Curran et al. (2004) |
| acsA-R | ACGAAGCGGTCATGGTC | Present work | ||||
| aroE, amp | aroE-F | CACTCCGGCAAAGGAACGA | 867 | 127-993bp | 55 | Present work |
| aroE-R | CTCAAATGCCGCCTGACAAC | Present work | ||||
| aroE, seq | aroE-F | TGATCCACCGCCTGTTCG | 676 | 53-728bp | 50 | Present work |
| aroE-R | ACCAGCATGCCCAGGC | Present work | ||||
| guaA | guaA-F | CGACAAGGTCACCGAGATGC | 652 | 432-1083bp | 57 | Present work |
| guaA-R | GACGTTGTGGTGCGACTTGA | Present work | ||||
| mutL | mutL-F | GCGACCTGTTCTTCAACAC | 530 | 455-984bp | 53 | Present work |
| mutL-R | GGTGCCATAGAGGAAGTCAT | Present work | ||||
| nuoD | nuoD-F | TCGATCCCTACTCCCTGTCC | 600 | 521-1120bp | 53 | Present work |
| nuoD-R | CCAGCTTGTCCCAGCC | Present work | ||||
| ppsA | ppsA-F | GGCCAAGCAGGCCAT | 578 | 891-1468bp | 50 | Present work |
| ppsA-R | GRTTGCCGACGTTCATCAT | Present work | ||||
| trpE | trpE-F | ATCAAGGACTACATCCTGGC | 727 | 697-1423bp | 55 | Present work |
| trpE-R | TGATGGTTTCTTCCCACTCC | Present work |
AT: annealing temperature; bp: base pairs; amp: amplification; seq: sequencing; Nt: nucleotide, referring to numbers of gene sequence of PAO1 (Genebank NC_002516.2).