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. Author manuscript; available in PMC: 2016 Sep 19.
Published in final edited form as: Sci Transl Med. 2011 Jun 22;3(88):88ra54. doi: 10.1126/scitranslmed.3002103

Fig. 4.

Fig. 4

Quantitative analysis of retinal transduction with AAV2 and AAV8. (A and B) Whole-mount retinal fluorescence after necropsy. Eyeballs were fixed, and the cornea, lens, and vitreous humor were removed to expose the posterior eye cup. Relative fluorescence was measured in a Xenogen Lumina IVIS imager and normalized to the fluorescence signal from an uninjected control eye. Eyes injected with 150 μl of 1010 genome copies (A) and 1011 genome copies (B) of AAV2 or AAV8 vector are shown. (C and D) Morphometric analysis of RPE and photoreceptor (PR) transduction by AAV2 and AAV8. Relative intensity (C) and relative area (D) of the GFP expression signal in RPE and PR were established at doses of 109 and 1010 genome copies based on morphometric histological analysis within the vector-exposed area. Numbers shown identify the animal used. L, left eye; R, right eye.