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. Author manuscript; available in PMC: 2016 Sep 19.
Published in final edited form as: Sci Transl Med. 2015 May 6;7(286):286ra68. doi: 10.1126/scitranslmed.3010658

Fig. 3. Antibiotic treatment of rats with anastomosis plus devascularization.

Fig. 3

Antibiotic treatment (Abx) of rats with anastomosis plus devascularization (Anast + Dvasc) attenuated MMP9 activation, and expression of HIF1-α (hypoxia inducible factor), iNOS (inducible nitric oxide) and MPO (myeloperoxidase). (A) Western blot analyses of rat tissues subjected to devascularization (Dvasc), anastomosis (Anast) or Anast + Dvasc, in the presence or absence of direct topical application of ciprofloxacin, metronidazole, and neomycin (topical Abx) via enema. Experiments were performed on 3 rats per group; displayed immunoblot is representative of all results. (B) The evaluation of band intensities using Image J software demonstrated the abundance of the active form of MMP9 in the rat group Anast + Dvasc, and loss of active MMP9 after topical application of antibiotics. n=3, p<0.01, Student t-test. (C) Zymography analysis confirmed the abundance of the active form of MMP9 in the Anast + Dvasc rat group, and loss of the active form of MMP9 by topical application of antibiotics.