Table 1. Examples of transgenic fly lines that can be used to label cargoes fluorescently in wing neurons.
| Strain | Purpose | BDSC No.(a) | Reference |
|---|---|---|---|
| Drivers: | |||
| Appl-Gal4 | Pan-neuronal GAL4 expression (chemosensory and mechanosensory wing neurons) | 32040 | (40) |
| dpr-Gal4 (b) | GAL4 expression in chemosensory neurons | 25083 | (14) |
| elavC155-Gal4 | Pan-neuronal GAL4 expression (chemosensory and mechanosensory wing neurons) | 458 | (41) |
| ok371-Gal4 | GAL4 expression in glutamatergic neurons | 26160 | (42) |
| nSyb-lexA | Pan-neuronal LexA expression (chemosensory and mechanosensory wing neurons) | 52817 | (43) |
| Fluorescent reporters (c): | |||
| UAS-ANF::EMD | Marks dense-core vesicles | 7001 | (7) |
| UAS-App::YFP | Marks APP-positive vesicles | 32039 | (8) |
| UAS-Eb1::GFP | Marks growing plus ends of microtubules | - | (44) |
| UAS-GalT::RFP | Marks Golgi vesicles | 30908 | Donated to BDSC by J. Lippincott-Schwartz |
| UAS-GCaMP5G | Intracellular Ca2+ reporter | 42037 | (45) |
| UAS-GCaMP6F | Intracellular Ca2+ reporter | 42747 | (28) |
| UAS-GFP | Marks entire cytoplasm | 6658 | (46) |
| UAS-mCD8::GFP | General marker of membranes | 5130 | (47) |
| UAS-mCD8::ChRFP (d) | General marker of membranes | 27392 | (48; donated to BDSC by F. Schnorrer) |
| UAS-mCD4::tdTomato | General marker of membranes | 35837; 35841 | (49) |
| UAS-mito::GFP | Marks mitochondria | 8442; 8443 | (9) |
| UAS-mCherry::mito (e) | Marks mitochondria | - | This study |
| UAS-Rab4::RFP | Marks Rab4-positive endosomes | 8505 | (50) |
| UAS-RedStinger::NLS | Marks nuclei | 8546 | (51) |
| lexAop-GFP | Marks entire cytoplasm | - | (43) |
| lexAop-mCherry::mito (e) | Marks mitochondria | - | This study |
| Other | |||
| tub-Gal80ts | Temperature sensitive version of GAL4 repressor | 7016 | (52) |
a
BDSC, Bloomington Drosophila Stock Center. See BDSC website for full genotypes;
b
contains a closely linked UAS-GFP transgene (see Fig. 5b);
c
UAS and lexAop lines are used with GAL4 and LexA, respectively;
d
also forms some cytoplasmic aggregates even in young flies;
e
unlike mito::GFP, in which GFP is targeted to the mitochondrial matrix using a sequence from human cytochrome C oxidase subunit VIII, mCherry::mito is anchored on the mitochondrial outer membrane using the Drosophila Miro transmembrane domain.