Figure 1.

Detection of orexin receptors expression in porcine preadipocytes. (a) OXR1 (white bars) and OXR2 (black bars) mRNA expression was analyzed one, three, or six days of differentiation. Results are shown as mean ± SEM, derived from n = 5 replicates. The experiment was repeated three times independently. (b) Western blot detection of OXR1 and OXR2 in preadipocytes differentiated for six days. Results are shown as mean ± SEM, derived from n = 3 replicates obtained from three independent preadipocytes isolations