Figure 2.

Changes in gene expression and SIR-2.1 protein expression of Caenorhabditis elegans (N2) fed on NGM/FUdR plates containing RES or OXY. The expression level of the genes was used to evaluate the effect of RES and OXY on the lifespan of C. elegans (a). Western blot was conducted to investigate the effect of OXY on SIR-2.1 protein expression (b, c). The expression level of SIR-2.1 protein significantly increased in the 1000 µM RES and 100, 500, and 1000 µM OXY groups, as compared with the control, in a dose-dependent manner. Asterisks denote significant differences compared with worms fed on NGM/FUdR plates containing DMSO only. *P < 0.05; **P < 0.01; ***P < 0.001