Skip to main content
. 2016 Sep 8;1(14):e88730. doi: 10.1172/jci.insight.88730

Figure 1. Correction of chloride (Cl–) transport in cystic fibrosis (CF) pig primary epithelial with a lentiviral vector.

Figure 1

Feline immunodeficiency virus–based viral vector expressing cystic fibrosis transmembrane conductance regulator (FIV-CFTR; MOI = 5) was delivered to the apical surface of well-differentiated primary cultures of airway epithelial cells from CF pigs. Transepithelial Cl– currents were measured in Ussing chambers. (A) An example of CFTR-dependent Cl– current is shown. The average change in Cl– current upon addition of forskolin/3-isobutyl-1-methylxanthine (F&I) (B) and GlyH-101 (C) in treated and naive cultures are shown. n = 8 epithelial sheets/treatment (collected from 3 donor pigs). *P < 0.01, Mann-Whitney nonparametric t test. (D) IHC using a CFTR antibody reveals apical localization of CFTR protein in ciliated cells (arrows). c, ciliated cells; nc, nonciliated cells. (E) IHC using a CFTR antibody on untreated CF primary airway epithelia. Scale bar: 250 μM (D and E).