Table 1.

Statistical summary of metabolomic profile of hindbrains of twitcher and WT control mice from a total of 314 named metabolites

2-way ANOVA comparisons	$\frac{\text{WT}\mathrm{p}22}{\text{WT}\mathrm{p}15}$	$\frac{\text{TWI}\mathrm{p}22}{\text{TWI}\mathrm{p}15}$	$\frac{\text{TWI}\mathrm{p}15}{\text{WT}\mathrm{p}15}$	$\frac{\text{TWI}\mathrm{p}22}{\text{WT}\mathrm{p}22}$
total metabolites (p≤0.05)	149	151	12	52
metabolites ( )
total metabolites (0.05<p<0.10)	17	17	10	15
metabolites ( )

A total of 314 metabolites were identified in the hindbrain specimens, and two-way analysis of variance (ANOVA) with post-test comparisons revealed varying numbers of statistically significant differences across the various comparisons of both genotype and time point (See also Supplementary Table 1), with statistical significance (p≤0.05). Comparison of the WT brain profiles on P15 and P22 indicated that almost half - 149 out of 314 - of the metabolites were significantly altered and the same was true of the twitcher brains, with 151 out of 314 changing significantly between P15 and P22. At P15, only 12 compounds exhibited statistically significant changes between WT and twitcher mice, whereas this number rose to 52 at P22.