Table 1.
Primary Antibodies Used
| Antigen | Description of Immunogen | Source, host species, catalog No., clone or lot No., RRID | Concentration used |
|---|---|---|---|
| Insulin‐like growth factor 2 Receptor (IGF2R/CI‐M6PR/CD222) | Synthetic peptide corresponding to residues surrounding Phe1379 of human IGF‐II receptor | Cell Signaling Technology, rabbit monoclonal, 15128 | 1:100 (immunostaining) 1:1,000 (Western blotting) |
| Leukocyte common antigen Ly5.1 (CD45.1) | SJL mouse thymocytes and splenocytes | Bio Legend, mouse monoclonal, 110708 RRID:AB_313497 | 2 µg/ml/106 cell (flow cytometry) |
| Leukocyte common antigen Ly5.2 (CD45.2) | B10.S mouse thymocytes and splenocytes | BD Biosciences, mouse monoclonal, 561874, RRID:AB_10894189 | 2.5 µg/ml/106 cell (flow cytometry) |
| CD11b/Mac‐1a | Mouse splenic cells CD11b | BD Biosciences, rat monoclonal, 553312, clone M1/70, RRID:AB_398535 | 2 µg/ml/106 cell (flow cytometry) |
| Lymphocyte antigen Ly‐6A/E (Sca‐1) | IL‐2‐dependent mouse T cell line CTL‐L | BD Biosciences, rat monoclonal, 561021, RRID:AB_2034021 | 2 µg/ml/106 cell (flow cytometry) |
| SCFR; stem cell factor receptor CD117 (cKit) | Mouse bone marrow mast cells | BD Biosciences, rat monoclonal, 561074, RRID:AB_10563203 | 2 µg/ml/106 cell (flow cytometry) |
| Lineage antibody cocktail, with isotype control | mouse T‐cell receptor (CD3e), mouse splenic cells (target CD11b); mouse abelson leukemia virus‐induced pre‐B tumor cells (target B222); mouse fetal liver (target Ter119, erythroids marker); Ly6G/6C | BD Biosciences, hamster antibody cocktail, 561301, RRID:AB_10611731 | 20 µl/100 µl/106 cell (flow cytometry) |