Figure 2.

Micro-porous scaffolds implanted for 30 days prior to tumor inoculation recruit metastatic cells. Number of (A) total cells and (B) tumor cells (tdTomato+ cells) isolated from micro-porous PLG and PCL scaffolds at day 15 post tumor inoculation analyzed via flow cytometry (N = 10, *p < 0.01 as determined by t-test for analysis of total cell numbers and Wilcoxon rank-sum test for tumor cell numbers). Fluorescence image of a PCL scaffold section shows the presence of a tumor cell (indicated by white arrow) as identified using tdTomato (C) and DAPI (D) fluorescence and their co-localization (E). Scale bar indicates 20 μm. Error bars denote s.e.m.