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. 2016 Sep 19;11(9):e0163335. doi: 10.1371/journal.pone.0163335

Fig 6. Molecular basis of the 'Sangiovese’ (SGM) cv. specific polymorphism.

Fig 6

(A) Sequence alignment of portions of the β-tubulin genes of three wine making cvs (‘Sangiovese’, ‘Croatina’, ‘Nebbiolo’), with the corresponding entry 8 of the Vitis vinifera Genome Data Base (VvGDB, version Genoscope 12x). Different molecular clones of the same cv. are indicated by different numbers. A 10 bp deletion is found in the SGM-17 clone. The arrows underscore the sequences used to design the forward and reverse primers and the star represents the fluorophore marker linked at 5’ position. (B) Portion of the electropherogram obtained by resolving, through capillary electrophoresis, the size of genomic DNA fragments amplified with the primer pair reported in (A). A specific doublet of 133–143 bp is detected only in the ‘Sangiovese’ (SGM) clones.