Fig 2. Barrier permeability assay with or without acrolein exposure.

Tissues were incubated with acrolein as the concentration indicated for 3 hours. (A). The TEER values were assessed by Ussing chamber system and associated voltage clamp. (B). The epithelial permeability was measured by fluorescent marker NaFI. Index of permeability represents the percentage of fluorescent marker passing though epithelium. Data represent Mean ±SE, n = 7, *: p < 0.05 as compared to controls.