Table 3. Antioxidant activity of MSE and fractions using ABTS•+ and DPPH method.
Antioxidant activity | ||
---|---|---|
Groups | IC50 DPPH (mg/mL) | ABTS•+ (μmol Trolox/g) |
MSE | 2.62±0.08a | 0.34±0.03a |
HF | 6.01±1.78b | 0.25±0.02a |
CLF | 1.78±0.20c | 0.64±0.10a |
EAF | 0.94±0.04d | 1.10±0.20b |
AF | 1.01±0.06d | 1.30±0.20b |
Reference values: BHT (0.1 mg/mL)__ 1268± 15 μmol Trolox/g (ABTS•+), (184 ± 2.6 μmol Trolox/g (DPPH). Averages of triplicates ± SD/means followed by letters showing the columns that differ statistically (p<0.05). Tukey's multiple comparison test. DPPH: 2,2-diphenyl-1-picryl-hydrazine; ABTS: 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, BHT (butilhidroxitolueno).