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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Neurosci Res. 2016 Nov;94(11):1138–1151. doi: 10.1002/jnr.23882

Figure 1. AAV construct designs and Outline of the study.

Figure 1

(A) The expression cassette for the traditional single stranded-AAV included a codon optimized and myc tagged mouse GALC gene under a Chicken Beta Actin (CBA-CAGGS) promoter along with SV40 poly-adenylation signal. For the self-complementary AAV design, a reduced mGALCopt-myc gene starting with the 2nd start codon is cloned under a minimal JeT promoter with a short synthetic polyA. (B) Time line for the interventions in the twitcher mice for the experiments outlined in the study.