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. Author manuscript; available in PMC: 2016 Sep 19.
Published in final edited form as: Methods Cell Biol. 2015 Mar 7;127:189–201. doi: 10.1016/bs.mcb.2015.01.002

Figure 1. Sample preparation for imaging IFT in mammalian primary cilia.

Figure 1

(A) The transwell cup is placed upside down in a humid sterile chamber. (B) Put 50 μL of the cell suspension (1 × 104 cells) on the membrane of the inverted transwell cup. (C) Culture cells for 3 hours at 37°C in 5% CO2. (D) The transwell cup is transferred to 24-well plate and media add into the transwell cup and the 24-well. (E) Culture cells until 90% confluency. (F) Put the transwell cup on a 35-mm glass bottom dish for imaging. We call it transwell imaging chamber.