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. Author manuscript; available in PMC: 2016 Sep 19.
Published in final edited form as: Methods Cell Biol. 2015 Mar 7;127:189–201. doi: 10.1016/bs.mcb.2015.01.002

Figure 3. Kymograph analysis for measuring IFT velocity.

Figure 3

(A) An image of IFT-88EGFP in the primary cilium of mIMCD-3 cells from Movie 1. (B) Kymographs of IFT-88EGFP moving in the cilium of mIMCD-3 cells. The kymographs was assembled from Movie 1. Bars: 5 s (horizontal), 5 μm (vertical). (A’ and B’) Draw lines along with the cilium or trajectories of IFT trains for making a kymograph and measuring the angle of the trajectory, respectively. (C) The IFT velocity is given from the angle of trajectory of IFT train.