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SDS-PAGE gel of nickel-affinity purified BmObc1 protein stained with Coomassie blue showing >90% purity. Aliquots from each step of the purification process were fractionated onto a 4–15% acrylamide gel. Lane (A) crude extract; (B) cleared lysate; (C) flow through; (D) wash 1 (E) wash 2; (F) eluted fraction; (G) protein standards
