Skip to main content
. Author manuscript; available in PMC: 2016 Sep 19.
Published in final edited form as: J Integr Plant Biol. 2016 Feb 5;58(4):426–439. doi: 10.1111/jipb.12446

Figure 3. miR825/825* overexpression plants are more susceptible to Pst DC3000.

Figure 3

(A) Expression of miR825 and miR825* in overexpression transgenic plants are examined by Northern blotting assay. RNA blots are hybridized with DNA oligonucleotide probes complementary to the indicated miRNAs. U6 is used as a loading control. (B) Phenotype of transgenic plants overexpressing miR825/825* (line 50 and 56) are compared to Col-0 WT plants. Pictures are taken 4 weeks after germination. (C) Expression of miR825 and miR825* target genes in the indicated transgenic lines is examined by qRT-PCR assay. Target genes and line numbers (#50 and #56) are as indicated. (D) AR156-mediated ISR in Col-0 and transgenic lines. Plants pre-treated with AR156 at 5 × 107 CFU/mL and 0.85% NaCl for 7 days, respectively. Leaves are spray inoculated with Pst DC3000 cell suspension at 1 × 108 CFU/mL concentration. ISR is measured by counting colonies on plates 3 days after inoculation (dpi). Error bars represent standard deviation for at least 12 leaf discs. Statistical difference among samples is represented by using letters: different letters (A, B, and C) represent significant difference (P < 0.01) while same letters represent insignificant differences (P > 0.01).