Figure 1. Spindle defects and chromosome misalignment in oocytes from diabetic mice.
Ovulated MII oocytes from control and diabetic mice were stained with β-tubulin antibody to visualize the spindle (green) and counterstained with TO-PRO to visualize chromosomes (red). MII oocytes from control mice present a typical barrel-shape spindle and well-aligned chromosomes on the metaphase plate. In MII oocytes from diabetic mice, spindle defects (arrows) and chromosome misalignment (arrowheads) were readily observed. Representative confocal sections are shown. Scale bars: 20 µm. (From Wang Q, et al. Maternal diabetes causes mitochondrial dysfunction and meiotic defects in murine oocytes. Mol Endocrinol 2009, 23:1603–1612, with minor modifications.)