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. Author manuscript; available in PMC: 2016 Sep 19.
Published in final edited form as: Mol Cell Biochem. 2013 Jan 30;376(1-2):163–173. doi: 10.1007/s11010-013-1564-3

Figure 2. A graphical representation of the chimeric protein strategy.

Figure 2

Using fusion PCR primers, each of these four constructs were developed and cloned into mammalian expression vectors: Slc2a9A/Slc2a1, Slc2a9A/Slc2a3, Slc2a9B/Slc2a1, and Slc2a9B/Slc2a3. In addition, the wildtype constructs Slc2a9A and Slc2a9B are displayed.