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. Author manuscript; available in PMC: 2016 Sep 19.
Published in final edited form as: Mol Cell Biochem. 2013 Jan 30;376(1-2):163–173. doi: 10.1007/s11010-013-1564-3

Figure 3. Localization of hGLUT9A in overexpressing MDCK cells (A) compared to cells overexpressing hGLUT9A with the N-terminus LL→AA Mutation (B).

Figure 3

hGLUT9A cDNA with the WT dileucine motif was cloned into a mammalian expression vector and stably overexpressed in MDCK cells. Immunofluorescent staining with a polyclonal antibody to the C-terminus reveals a basolateral membrane sorting pattern (A). The same pattern is seen in the construct containing a LL→AA mutation in the N-terminus (B).