Skip to main content
. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Mol Neurobiol. 2016 Mar 19;54(4):2659–2673. doi: 10.1007/s12035-016-9851-0

Figure 4. The BoNT/B and BoNT/A treatments abolish the MSC-exosomes increased axonal growth.

Figure 4

Quantitative data (A) show the effect of naïve exosomes (exo) and exosomes pretreated with BoNT/B (BoNT/B-Exo) on distal axonal length when the exosomes were applied into the soma (Soma) or the axonal compartment (Axon). Data were normalized to the control group (Ctl). *p<0.05 vs control and #p<0.05 vs naïve exosomes. Representative Western blot images and quantitative data (B) show levels of VAMP2, Alix and Hsp70 proteins in naïve exosomes (Exo) and exosomes pretreated with BoNT/B (BoNT/B). *p<0.05 vs naïve exosomes. Panel C shows the effect of naïve MSC-exosomes on axonal length of the cortical neurons pretreated with BoNT/A (BoNT/A+Exo) for 24h in the cell body (Soma) or the axonal compartment (Axon) compared to the control (Ctl), BoNT/A pretreatment only (BoNT/A) and exosome only (Exo) groups. *p<0.05 vs control and #p<0.05 vs exosome only. Quantitative data of axonal elongation in the axonal compartment (D) show the effect of the naïve exosomes (Exo) on distal axonal growth of cortical neurons with BoNT/A pretreatment (BoNT/A+Exo) or without BoNT/A pretreatment (Exo) during 60 min of time-lapse microscopic experiments when MSC-exosomes were applied into the somal (Soma, n=45/Exo and 41/BoNT/A+Exo) or axonal (axon, n=44/Exo and 40/BoNT/A+Exo) compartment. *p<0.05 vs exosomes. Representative Western blot images (E) and quantitative data (F) show the effect of BoNT/A treatment on soma and axonal SNAP25 proteins when BoNT/A was applied to the cell body (BoNT/A, Soma) and axonal (BoNT/A, Axon) compartments, respectively, compared to non-treated neurons (Ctl, Soma) and axons (Ctl, Axon). *p<0.05 vs control. Representative microscopic images (G) and quantitative data (H) show the effect of BoNT/A (BoNT/A) treatment on uptake of FM1-43FX dye by axonal growth cones. *p<0.05 vs control (Ctl).