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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Cell Physiol. 2016 Apr 26;232(1):182–191. doi: 10.1002/jcp.25406

Figure 3.

Figure 3

Upregulation of Dicer expression by Runx2 overexpression. (A) (a) Runx2 and DICER protein were detected by Western blot; (b) mRNA levels of Runx2 and DICER were detected by qRT-PCR in C3H10T1/2 cells. The cells were induced in ostegenic medium for 4 days, and collected 48 hours after Runx2 cDNA or empty vector transfection. (B) (a) DICER protein was detected by Western blot in MC3T3-E1 cells. The cells were induced in ostegenic medium for 0 and 4 days, respectively, and collected 48 hours after Runx2 cDNAs or empty vector transfection. (−), untransfected cells as a internal control. (b) mRNA levels of Runx2 and DICER were detected by qRT-PCR in MC3T3-E1 cells transfected with Runx2 cDNAs or empty vector. The cells were induced in ostegenic medium for 4 days. These data are expressed as the mean ± SD (n=3). * p < 0.05 versus empty vector group.