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. Author manuscript; available in PMC: 2017 Sep 22.
Published in final edited form as: Vaccine. 2016 Aug 28;34(41):4969–4978. doi: 10.1016/j.vaccine.2016.08.041

Fig. 3. Immunization with rLVS ΔcapB overexpressing FPI T6SS proteins induces greater T-cell mediated immune response and a Th1-type antibody response.

Fig. 3

Mice (3/group) were immunized i.d. with various vaccines; euthanized 4 weeks later; and their splenocytes isolated and stimulated with IglC or HI-LVS overnight or for 3 days. The culture supernatant was assayed for IL-17A (A) or IFN-γ (B) after 3 days incubation with antigen and the cells assayed for CD4+ or CD8+ T cells expressing IFN-γ or IFN-γ +TNF as indicated after overnight incubation with antigen (D–F). Their sera were isolated and assayed for antibodies specific to HI-LVS (C). Values are means ± SE. *, P<0.05; **, P < 0.01; ***, P< 0.001; ****, P< 0.0001 by Two-Way ANOVA with multiple comparisons (Prism 6.04); all comparisons are between the vaccine directly beneath the open bar and the other vaccines. The results from the experiment shown are representative of two independent experiments.