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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Insect Biochem Mol Biol. 2016 Aug 12;77:69–77. doi: 10.1016/j.ibmb.2016.08.003

Figure 4.

Figure 4

(A) qPCR analysis using Gal4 or EGFP primers showing the midgut-specific expression of ET-Gal4>UAS-EGFP transgene at 72 h PE. ND means non-detected. RNA samples were extracted from midgut (MG), fat bodies (FB), ovaries (OV), and Malpighian tubules (MT) at 72 h PE. Analyses were performed by means of qPCR using specific probes (Table S2). (B) Topical JH application induces premature expression of EGFP in midgut (MG) of the ET-Gal4>UAS-EGFP transgenic line (JH). Application of acetone (solvent) served as a control. Images were obtained using a Leica M165FC fluorescent stereomicroscope with LAS V4.0 software. Scale bar: 1 mm.