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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Insect Biochem Mol Biol. 2016 Aug 12;77:69–77. doi: 10.1016/j.ibmb.2016.08.003

Figure 5.

Figure 5

Depletion of Met by RNAi results in inhibitory effects on the expression of endogenous ET and ET-Gal4>UAS-EGFP transgene. (A) Knockdown of Met by RNAi results in reduced transcript levels of endogenous ET and ET-Gal4>UAS-EGFP transgenes. The abundance of dsRNA-injected samples is represented as 1.0, with corresponding adjustments for other time points. Values represent average ± s.e.m. from three combined biological replicates. *p < 0.05; **p < 0.01; ***p < 0.001 (t-test) (B) The EGFP signal was significantly reduced in midguts (MG) of dsMet-treated female mosquitoes compared with dsLuc-treated female mosquitoes. WT mosquitoes treated with dsLuc or dsMet served as negative controls. Images were obtained using a Leica M165FC fluorescent stereomicroscope with LAS V4.0 software. Scale bar: 1 mm.