Fig. 1.

Characterisation of YS- and BM-derived KCs. (A) Experimental approach used to generate irradiation chimeras with GFP⁺ YS-derived KCs. (B) ALT and (C) AST levels in the serum of mice at different time points post-irradiation as compared to control (non-irradiated) animals. (D) H&E stained sections from the liver of control non-irradiated mice or mice (E) 24 h (F) 3 days or (G) 7 days post-irradiation demonstrating very little liver damage or inflammation as a result of irradiation. (H) Immunofluorescent image demonstrating the presence of GFP⁺ F4/80⁺ liver resident KCs and GFP^- F4/80⁺ BM-derived KC in the livers of the chimeras generated in (A) (left) or reciprocal chimeras (right), 6 weeks post-irradiation. GFP (green), F4/80 (red). (I) Flow cytometry analysis of the livers of chimeras generated via the method shown in (A) gated on forward scatter (FSC) and side scatter (SSC), CRIg and F4/80 expression and GFP. (J) Analysis of the percentage of CRIg⁺ F4/80⁺ cells that express GFP (left) and the number of CRIg⁺ F4/80⁺ GFP⁺ cells in the liver (right) over time. Symbols represent individual mice and are representative of 2 experiments with 5-6 mice per group. (I) The volume and surface area of KCs in 3 dimensions. (L) 2-photon intravital imaging of YS-derived KCs in the livers of chimeras generated via the method shown in (A) (top) or the reciprocal chimera (bottom). Data were analysed with a non-parametric t test. ∗p <0.05.