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. 2016 Sep 20;7:1401. doi: 10.3389/fpls.2016.01401

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Copyright © 2016 Latarullo, Tavares, Padilla, Leite and Buckeridge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biomass units of sugarcane stem (culm) with cells bound by middle lamellae. (A,C) Stele, the vascular bundle showing Fibers (F), Phloem (Ph), Xylem (X), sucrose-storage parenchyma (sP). (B) Macerated tissue showing a detail of an isolated stele that still maintains adhesion among cells. (D) Cells of sugarcane stem without the binding of middle lamellae. Cells are named as in (A). The cell separation was prepared according to Franklin (1945) and cells were stained with toluidine blue (B,D) and iodine-potassium iodide (A,C). Bars represent 100 μm.