Table 2.

Primer sequences used to validate the gene transcription levels obtained by RNA-seq approach.

Locus ID	Gene/ORF name	Sequence primers (5′–3′)
BF9343_0382	gadB	F-TGGCAGATTGAGATGCGTCA
		R-CAGACCGGTCCATGTAACCC
BF9343_3128	BF9343_3128	F-CTCATCCATCCTGACCGACG
		R-GCAGTTCGGAGTTTTGCTGG
BF9343_2131	pyrD2	F-CAGGGTTGGCAGTTTTTCG
		R-ACCCGGTACAGACGAGTACA
BF9343_3319	gdhB2	F-TCTCCACGCGGAAAGTCAAA
		R-GTCGGTTTCAGGACCGATGT
BF9343_3934	BF9343_3934	F-TGTCACTGAATTGAGGGCGT
		R-ACCATCCGAAAGACAGACCG
BF9343_0081	BF9343_0081	F-CACATGTCCATAGCCGTCGT
		R-CTGATCGCCCTTCATCCACA