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. 2016 Aug 1;10(8):ZC28–ZC32. doi: 10.7860/JCDR/2016/19007.8236

[Table/Fig-4]:

H & E staining steps.

100% Alcohol Slide was dipped for 1 minute
90% Alcohol Slide was dipped for 1 minute
80% Alcohol Slide was dipped for 1 minute
Tap water 1 dip
Hematoxylin 3 minutes
Tap water 1 dip
1% Acid Alcohol 1 dip
Blueing 15 minutes
Eosin 2 minutes
80% Alcohol 1.5 minute
90% Alcohol 1.5 minute
100% Alcohol 2 minute
Xylene After drying, clearing for 15 minutes
DPX Mounting