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. 2016 Sep 20;6:33605. doi: 10.1038/srep33605

Figure 2. Protein-up-regulating activity of R12A-AS1 depends on the overlap with the mRNA and FRAM.

Figure 2

(a) Western blot analysis of PPP1R12A in HEK293T cells transfected with the indicated R12A-AS1 clones (60 and 120 pmol) or empty vector as control. Lower panel shows mean PPP1R12A band intensity, as in Fig. 1d. (b) RNA levels, as in Fig. 1e. (c) Western blot analysis of PPP1R12A in HEK293T cells transfected with 30, 60 and 120 pmol of pR12A-AS1 either without or with the FRAM ED, or empty vector as control. Lower panel shows PPP1R12A band intensity, normalized to actin B. (d) The levels of indicated RNAs, detected by qPCR. (e) Western blot analysis of PPP1R12A in HeLa cells transfected with pR12A-AS1 or empty vector as control. Lower panel shows PPP1R12A band intensity, as in Fig. 1d. (f) RT-qPCR analysis of RNA levels. *(n = 3, mean + S.D., p < 0.05, One-Sample t-Test, vs empty vector).