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. 2016 Sep 13;4:e2423. doi: 10.7717/peerj.2423

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© 2016 Amarillas et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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PCR was performed to detect the presence of stx₁ and stx₂ genes in phage genome. The size of stx₁ and stx₂ amplicon corresponds to the 180 and 255 bp band, respectively. Lane M; 100 bp DNA ladder (Promega), Lane 1; negative control, Lane 2; Bacteriophage phiC119 sample, Lane 3; positive control.