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. 2016 Sep 20;6:33671. doi: 10.1038/srep33671

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Copyright © 2016, The Author(s)

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Ubiquitin or K48-linked diubiquitin (Ub₂) were added by syringe to Ddi1 constructs in the sample cell. The different titrations were (a) ubiquitin to RVP-UBA, (b) ubiquitin to UBL-RVP, (c) ubiquitin to full-length Ddi1, and (d) K48-Ub₂ to full-length Ddi1. For full-length Ddi1 binding to ubiquitin (c), the data was also fitted to a model with two independent binding sites (K_d1 and K_d2), each with a stoichiometry of 1.