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. 2016 Sep 15;4:e2461. doi: 10.7717/peerj.2461

Figure 2. Debcl-induced phenotypes are rescued by the pro-survival Buffy.

Figure 2

(A) The directed inhibition of debcl in the DA neurons driven by Ddc-Gal4 results in a slightly increased median survival compared to the control flies overexpressing UAS-lacZ, while the overexpression of debcl results in severely reduced survival. The genotypes are UAS-lacZ/Ddc-Gal4; UAS-debcl-RNAi/Ddc-Gal4 and UAS-debcl/Ddc-Gal4. Longevity is shown as percent survival (P < 0.01, determined by log-rank and n ≥ 200). (B) The inhibition of debcl results in improved climbing ability whereas the overexpression of debcl results in a highly compromised climbing ability as determined by non-linear fitting of the climbing curves and comparing at 95% confidence intervals. The genotypes are UAS-lacZ/Ddc-Gal4; UAS-debcl-RNAi/Ddc-Gal4 and UAS-debcl/Ddc-Gal4. Error bars indicate the standard error of the mean (SEM) and n = 50. (C) The overexpression of Buffy along with the overexpression of debcl or debcl-RNAi restores lifespan and (D) significantly improves the climbing ability of these flies. The genotypes are UAS-Buffy; Ddc-Gal4/UAS-lacZ, UAS-Buffy; Ddc-Gal4/UAS-debcl-RNAi and UAS-Buffy; Ddc-Gal4/UAS-debcl. Longevity was determined by log-rank (Mantel-Cox) test and n ≥ 200 while climbing ability curves were fitted non-linearly and compared with 95% CI.