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. 2016 Sep 14;4:e2451. doi: 10.7717/peerj.2451

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©2016 Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) The smooth-edged instrument made of bull-horn for Gua Sha treatment, with the size and shape similar as a credit card. (B) Gua Sha treatment on a person’s back. (C) The pattern of blemishes resulted from Gua Sha treatment in human. Mouse skin prior to Gua Sha treatment observed from the stratum corneum side (D), (blue arrow indicates the direction of Gua Sha operation) and the dermal side (G); mouse skin after 20 or 40 scrapes observed from the stratum corneum side (E & F) and the dermal side (H & I) . Photos were taken 30 min after Gua Sha treatment from the stratum corneum side, then the mice were euthanized for observation from the dermal side. Images are representative ones from three mice per group.