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. 2016 Sep 14;4:e2451. doi: 10.7717/peerj.2451

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©2016 Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Vaccination was performed 10 min after 20 or 40 scrapes at day 1, 21 and 42. Groups vaccinated with OVA and OVA + FIA in untreated mice were as the negative and positive controls, respectively. Sera were collected after prime, the first boost and the second boost (day 20, 41 and 55). IgG (A, B & C), IgG1 (D) and IgG2a (E) were determined with ELISA. Data were shown as mean ± SD. OVA-specific IgG1/IgG2a ratios of individual mouse were calculated using IgG2a responders. Data were shown as mean + SD (F) (n = 6; *, p < 0.05; **, p < 0.01; ***, p < 0.001; One-way ANOVA with Dunnett’s posttest.)