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Orthologous multiple sequence alignment of FOXD3 across species. Highly conserved regions are colored in blue. The winged helix-turn-helix DNA binding region spans a region beginning at position 71 and ending at position 173. This region is highly conserved. The single nonconserved residue within the DNA binding domain occurs at position 148 in the alignment where human, dog, and painted turtle have asparagine (N) while chicken, garter snake, anole lizard, and python have serine (S).
