Figure 5. Y₁R deletion impairs osteoclast resorption activity.

The generation of 3D reconstructions of WT (A) and Y₁R^−/− (B) resorption pits revealed striking morphological differences. The analysis of these 3D reconstructions demonstrated that Y₁R^−/− resorption pits exhibit a decreased top section area (C), pit size (D), depth (E) and volume (F). Moreover, an increase on Y₁R^−/− pit depth is not accompanied by augmented top section area (G) or volume (H). Although the mean aspect ratio of Y₁R^−/− resorption pits was not different from WT (I), the frequency distribution of Y₁R^−/− resorption pits revealed a shift towards less circular pits than WT (J), which did not alter with increases in pit volume (K). Data is expressed as mean ± SEM. ***p < 0.001 different from WT.