Table 1. Pairs of primers used in qRT-PCR analysis.
Gene | Forward Primer (5′- > 3′) | Reverse Primer (5′- > 3′) | Product Length (base pairs) |
---|---|---|---|
ATP6V0D2 | TTCTTGAGTTTGAGGCCGAC | CAGCTTGAGCTAACAACCGC | 144 |
CTSK | ATATGTGGGCCAGGATGAAAGTT | TCGTTCCCCACAGGAATCTCT | 90 |
CD47 | CGATGCCATGGTGGGAAACT | TCAGTGTTGAAGGCCGTGC | 99 |
CD9 | GCTGGGATTGTTCTTCGGGT | GCTTTGAGTGTTTCCCGCTG | 171 |
DC-STAMP | AAGCGGAACTTAGACACAGGG | CAGCTAGGGCTTCGTGGAAA | 101 |
GADPH | GCCTTCCGTGTTCCTACC | AGAGTGGGAGTTGCTGTTG | 183 |
MCP-1 | AGCCAACTCTCACTGAAGCC | GCGTTAACTGCATCTGGCTG | 131 |
MMP-9 | CGACTTTTGTGGTCTTCCCC | TAGCGGTACAAGTATGCCTCTG | 83 |
OPG | GTGGAATAGATGTCACCCTGTGT | TTTGGTCCCAGGCAAACTGT | 110 |
RANKL | CCCATCGGGTTCCCATAAAGT | AGCAAATGTTGGCGTACAGG | 114 |
TRAcP5b | CGACCATTGTTAGCCACATACG | TCGTCCTGAAGATACTGCAGGTT | 77 |
Y1R | CTCGCTGGTTCTCATCGCTGTGGAACGG | GCGAATGTATATCTTGAAGTAG | 325 |
All primers used were located on two different exons to ensure that only properly spliced mRNA and not genomic DNA contaminants was amplified.