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. 2016 Sep 20;6:33470. doi: 10.1038/srep33470

Table 1. Pairs of primers used in qRT-PCR analysis.

Gene Forward Primer (5′- > 3′) Reverse Primer (5′- > 3′) Product Length (base pairs)
ATP6V0D2 TTCTTGAGTTTGAGGCCGAC CAGCTTGAGCTAACAACCGC 144
CTSK ATATGTGGGCCAGGATGAAAGTT TCGTTCCCCACAGGAATCTCT 90
CD47 CGATGCCATGGTGGGAAACT TCAGTGTTGAAGGCCGTGC 99
CD9 GCTGGGATTGTTCTTCGGGT GCTTTGAGTGTTTCCCGCTG 171
DC-STAMP AAGCGGAACTTAGACACAGGG CAGCTAGGGCTTCGTGGAAA 101
GADPH GCCTTCCGTGTTCCTACC AGAGTGGGAGTTGCTGTTG 183
MCP-1 AGCCAACTCTCACTGAAGCC GCGTTAACTGCATCTGGCTG 131
MMP-9 CGACTTTTGTGGTCTTCCCC TAGCGGTACAAGTATGCCTCTG 83
OPG GTGGAATAGATGTCACCCTGTGT TTTGGTCCCAGGCAAACTGT 110
RANKL CCCATCGGGTTCCCATAAAGT AGCAAATGTTGGCGTACAGG 114
TRAcP5b CGACCATTGTTAGCCACATACG TCGTCCTGAAGATACTGCAGGTT 77
Y1R CTCGCTGGTTCTCATCGCTGTGGAACGG GCGAATGTATATCTTGAAGTAG 325

All primers used were located on two different exons to ensure that only properly spliced mRNA and not genomic DNA contaminants was amplified.