Figure 2.

Lgals1^−/− mice have a higher yeast burden in the lung and spleen day 15 after infection. On day 15 after infection with H. capsulatum (5 × 10⁵ viable yeasts), animals were sacrificed and tissue samples were harvested. (a) Lung sections (5 μm) from WT and Lgals1^−/− mice were stained with silver (Grocott's methanamine silver (GMS); bar: 50 μm; insert bar: 25 μm) and (b) yeast cells were quantified (yeast/mm² lung) using magnifications ×400. Fungal burden was quantified from tissue homogenates and expressed as the number of colony-forming units (CFU) per gram of tissue CFU/g in lung (c) and CFU per spleen (d). Data are representative of one of the two experiments performed independently (n = 10 per group). Values are mean ± SEM. ^# p < 0.05 WT versus Lgals1^−/−, both infected.