Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 20;6:33666. doi: 10.1038/srep33666

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Control GF (GF4) was infected with lentiviruses carrying artificial miRNAs together with EmGFP. Over 99% of lentivirus-infected cells were positive for EmGFP that indicate highly efficient transduction of artificial miRNAs. Control cells were transduced with negative control miRNA (NC miR). For knockdown of DLX5 and RUNX2 long form, 4 different miRNA sequences for each transcript were designed (DLX5 miR #1 to #4, and RUNX2 long miR #1 to #4). The pictures of fluorescence microscopy for GF4 infected with lentiviruses carrying NC miR, DLX5 miR #2, and RUNX2 long miR #1 are shown. Scale bar indicates 200 μm. (b) RT-qPCR for DLX5, RUNX2 long form transcribed from p1 RUNX2 promoter, and RUNX2 short form transcribed from p2 RUNX2 promoter. RNA was collected 5 d after lentiviral infection. The expression of each gene was normalized to that of GAPDH. Bars represent mean ± SD. (c) RT-qPCR for RUNX2 long form transcribed from p1 RUNX2 promoter and RUNX2 short form transcribed from p2 RUNX2 promoter. RNA was collected 5 d after lentiviral infection. The expression of each gene was normalized to that of GAPDH. Bars represent mean ± SD. (d) Venn diagram of down-regulated genes (fold change <0.5) by knockdown of DLX5 miR #2, #4 and RUXN2 long miR #1.