FIG. 7.

Rev and RRE contributions to functional activity in subtype G viruses. The activity of subtype G virus Rev–RRE cognate pairs as well as Rev–RRE pairs created by transposing the 8-G and 9-G Rev and RRE components were measured using the standard Rev sequences (A) or HA-tagged Rev sequences (B). Bars show average deviation from the mean for two replicates. (C) Western blot showing the level of steady state Rev expression of both subtype G HA-tagged Rev isolates. Rev expression was detected using a fluorescent secondary antibody and infrared imager. (D) Rev intensity values were measured and normalized to beta-tubulin to control for loading variation and to SEAP expression to control for transfection efficiency, yielding arbitrary normalized units. HA, hemagglutinin; SEAP, secreted embryonic alkaline phosphatase.